华中农业大学

宁国贵：男，1979年1月生，博士。华中农业大学副教授,硕士生导师。博士毕业论文题目为“矮牵牛不同花型突变体的形态结构及其分子基础分析”。主要研究领域：园林植物细胞与分子生物学。目前从事的研究领域具体涉及到(1)野蔷薇、月季和玫瑰等（蔷薇科Rosaceae）等木本植物(2)矮牵牛和烟草等（茄科Solanaceae）等草本植物的开花时间调控及开花过程中花色、花香等次生代谢物代谢方面的相关分子生物学研究。主要研究方法：生物信息分析与比较基因组，转基因分析重要功能候选基因，液相气质等测定分析代谢物等的研究方法。已在国内外发表论文近30篇。

Email: ggning@mail.hzau.edu.cn & whnobleman2004@yahoo.com.cn

TEL: 　027-87286928 (o)

主讲课程如下：

《园林植物育种学》、《花卉学》、《园林植物育种学实验》、《花卉学实验》、《园艺植物育种学》等课程。

主持与参加主要科研项目如下：

1.瑞典国际科学基金：Shorting juvenility of Platanus acerifolia and Prunus mume via transformation using FT orthologs（D/4349-1，项目负责人）2.国家自然科学基金：梅花开花时间相关基因的克隆及其功能解析（30800761，项目负责人）

3.国家自然科学基金： 月季及其近缘种MYB 不同类型转录因子的结构及功能研究（31171985，项目负责人）

4.科技部“十二五”863基金：月季、菊花、百合分子育种与品种创制（2011AA100208，月季子项目分子育种部分负责人）

5.华中农业大学科技创新基金：蔷薇属三物种花发育代谢物质的差异性及其与酶基因的相关性研究(2011PY103，项目负责人）

6.国家自然科学基金：早花变异枳LFY基因启动子结构及表达机制调控研究(30671434, 项目参加人)

7.国家自然科学基金项目：矮牵牛花器官发育的分子遗传分析及人工调控(30671477, 项目参加人)

8.教育部博士点基金：表皮毛发育相关基因对烟草与矮牵牛花青素含量调控的机理研究(2008-2010，项目参加人)

已发表的主要论文：

1.Guogui Ning*, Xiao Xiao, Haiyan Lv, Xin Li,Yang Zuo, Manzhu Bao*.Shortening tobacco life-cycle accelerates functional gene identification in genomic research. Plant biology 2012, In press (SCI)

2.Liao Liao, Cai-Xian Liu, ·Guo-Gui Ning,· Man-Zhu Bao. A pFBP6::BARNASE construct resulted in stigma and style ablation and floral abscission in transgenic tobacco. Plant molecular biology reporter 2012, In press (SCI)

3.Zhen Wang, Shafei Ye, Jingjing Li, Bo Zheng, Manzhu Bao, Guogui Ning*.Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning. BMC Biotechnology 2011, 11:109 doi: 10.1186/1472-6750-11-109(SCI)

4.Ying Bao Guofeng Liu Xueping Shi Wen Xing Guogui Ning Juan Liu Manzhu Bao Primary and repetitive secondary somatic embryogenesis in Rosa hybrida ‘Samantha’Plant cell, tissue &organ culture. 2011, In press (SCI)

5.Shijia Luo, Yanhong He, Guogui Ning, Jiaqi Zhang, Guangying Ma and Manzhu Bao. Genetic diversity and genetic structure of different populations of the endangered species Davidia involucrata in China detected by inter-simple sequence repeat analysis. Trees,2011, DOI: 10.1007/s00468-011-0581-7(SCI)

6.J. Zhang, G. Liu, C. Guo, Y. He, Z. Li, G. Ning, X. Shi & M. Bao. The FLOWERING LOCUS T orthologous gene of Platanus acerifolia is expressed as alternatively spliced forms with distinct spatial and temporal patterns. 2010, doi:10.1111/j.1438-8677.2010.00432.x(SCI)

7.Yanhong He,Guogui Ning*, Yalin Sun, Yanhu, Xingyu Zhao, Manzhu Bao. Cytological and mapping analysis of a novel male sterile type resulting from spontaneous floral organ homeoticconversion in marigold (Tagetes erecta L.). Mol Breeding,2010,DOI 10.1007/s11032-009-9372-x(SCI)

8.Wen Xing, Manzhu Bao, Hongdao Qin,Guogui Ning*. Micropropagation of Rosa Rugosa through axillary shoot proliferation.ACTA BIOLOGICA CRACOVIENSIA Series Botanica 2010,52/2: 75–56(SCI)

9.Shi XP, Dai XG, Liu GF, Zhang JW, Ning GG, Bao MZ. Cyclic secondary somatic embryogenesis and efficient plant regeneration in camphor tree (Cinnamomum camphora L.). In Vitro Cell Dev Biol Plant, 2010, DOI 10.1007/s11627-009-9272-0(SCI)

10.Guangying Ma, Guogui Ning, RuihuaPang, JingZhan, XinLi,WeiZhang, Manzhu Bao. Analysis ofthe Petunia hybrida double flowertranscriptomeusingsuppression subtractive hybridization. Scientia Horticulturae ，doi:10.1016/j.scienta.2010.10.003(SCI)

11.Guangying Ma, Guogui Ning, Wei Zhang, Jing Zhan, Haiyan Lv, Manzhu Bao.Overexpression of Petunia SOC1-like Gene FBP21 in Tobacco Promotes Flowering Without Decreasing Flower or Fruit Quantity. Plant Mol Biol Rep, 2010,DOI 10.1007/s11105-010-0263-4(SCI)

12.Wei Zhang, Guogui Ning, Haiyan Lv, Liao Liao, Manzhu Bao. Single MYB-type transcription factor AtCAPRICE: A new efficient tool to engineer the production of anthocyanin in tobacco. Biochemical and Biophysical Research Communications, 2009,388(4):742-747(SCI)

13.Y.H. He, G.G. Ning, Y.L. Sun, Y.C. Qi and M.Z. Bao.Identification of a SCAR marker linked to a recessive male sterile gene(Tems) and its application in breeding of marigold (Tagetes erecta L.). plant breeding,2009, 128: 92—96 (SCI)

14.G.G.Ning, X.P. Shi, H.R Hu, Y.Yan, M.Z. Bao. .Development of a range of polyploid lines in Petunia hibrida and the relationship of ploidy with the single-/ double-flower trait. Hortscience, 2009,44(2):250-255(SCI)

15.W.J. Huang, G.G. Ning, G.F. Liu, M.Z. Bao. Determination of genetic stability of long-term micro-propagated plantlets of Platanus acerifolia using ISSR markers. BIOLOGIA PLANTARUM ,2009,(1): 159-163 (SCI)

16.XiaoPeng Fu, GuoGui Ning, LiPing Gao, ManZhu Bao .Genetic diversity of Dianthus accessions based on molecular marker systems (SRAPs and ISSRs) and morphological traits. Scientia Horticulturae, 2008,113:263-270 (SCI)

17.Y. M. Ye J. W. Zhang G. G. Ning & M. Z. Bao. A comparative analysis of the genetic diversity between inbred lines of Zinnia elegans using morphological traits and RAPD and ISSR markers. Scientia Horticulturae, 2008,118:1–7(SCI)

18.G. G Ning, S. P Bai, M. Z Bao, L. Liu. Factors affecting plantlet regeneration from in vitro cultured immature embryos and cotyledons of Prunus mume ‘Xue Mei'. In Vitro Cell. Dev. Biol.-Plant. . 2007, 43: 95-100(SCI)

19.G.G Ning, M.Z Bao.Plant regeneration from callus derived from immature embryo cotyledons of Prunus mume. HortScience, 2007, 42(3): 744-747(SCI)

20.G.G NING, X.L FAN, W.J HUANG, M.Z BAO, J.B. Micropropagation of six Prunus mume cultivars through high-frequency axillary shoots proliferation and ISSR analysis of cloned plants. Acta Biologica Cracoviensia, 2007,49(1):25-31(SCI)

21.包颖，张蔚，邢文，宁国贵，包满珠* MYB家族基因CPC在月季中的遗传转化研究. 中国科技论文在线,2012,http://www.paper.edu.cn/index.php/default/releasepaper/content/201201-763

22.邢文,张蔚,包颖,宁国贵,包满珠*植物类黄酮生物合成的MYB转录因子研究进展.中国科技论文在线，2012,http://www.paper.edu.cn/index.php/default/releasepaper/content/201201-789

23.宁国贵，吕海燕，张俊卫，包满珠.梅花不同外植体离体培养及体细胞胚诱导植株再生. 园 艺学 报 2010， 1：114 – 120

24.王文恩,包满珠,张俊卫,刘国锋,宁国贵,尹少华.狗牙根辐射诱变后代变异植株的形态特征比较和ISSR分析. 草业科学，2009，26(12):139-145

25.吕海燕,宁国贵,胡惠蓉,包满珠.矮牵牛突变自交系离体培养及变异植株稳定性观察. 华中农业大学学报，2009 ,28(6):746～749

26.宁国贵，白三平，包满珠，黄文俊.矮牵牛细胞的长期离体培养及再生植株的 ISSR 分析. 中国农业科学.2007,40(7):1479-1485

27.齐迎春,宁国贵,包满珠.应用 ISSR 分子标记和表型性状评价孔雀草自交系的遗传关系. 中国农业科学.2007,40(6): 1236-1241

28.宁国贵,包满珠.矮牵牛一种新花器官突变体的表型与解剖结构特征分析.园艺学报,2006,5:1121-1124

29.宁国贵，代色平，包满珠，沈宝仙. 矮牵牛S4代13个自交系主要性状分析中南林学院学报，2003， 23（5）：76－79

目前主要专利申请：

1.农杆菌介导的玫瑰遗传转化方法（排名第一）

2.一种获得月季‘萨曼莎’转基因植株的方法（排名第一）

3.玫瑰再生为完整植株的方法（排名第一）

4.以未成熟种子为外植体的月季再生植株的方法（排名第五）

5.以叶片为外植体的月季再生植株的方法（排名第五）